Preparation of Research Manuscripts  Manuscript categories  Organization of the Manuscript
Authorship requirements Full-length paper Title Page
Author identification – ORCID Short communication  Running title 
Resubmissions Review article  Authors and Affiliations 
Plagiarism Overview Key Words
Publication charges Concepts and Comments  Abstract
Cover letter Case report  Introduction
Text format  Material and Methods
Footnotes Results
Headings in text  Discussion
Abbreviations Acknowledgments
Nomenclature Figures

Preparation of Research Manuscripts

The Brazilian Journal of Medical and Biological Research publishes original research articles of outstanding scientific significance. We will consider manuscripts of any length; we encourage the submission of both substantial full-length bodies of work and shorter manuscripts that report novel findings that might be based on a limited number of experiments. The key criteria are that the research demonstrates clearly its novelty, its importance to a particular field as well as its interest to those outside that discipline, and conclusions that are justified by the data.

Authorship requirements

Only those persons who contributed directly to the intellectual content of the paper should be listed as authors. Authors should meet all of the following criteria, thereby allowing persons named as authors to take public responsibility for the content of the paper.

  • Conceived, planned and carried out the experiments that led to the paper or interpreted the data it presents, or both.
  • Wrote the paper, or reviewed successive versions.
  • Approved the final version.

Holding positions of administrative leadership, contributing patients, and collecting and assembling data, however important to the research, are not by themselves criteria for authorship. Other persons who have made substantial, direct contributions to the work but cannot be considered authors should be cited in the Acknowledgment section, with their permission, and a description of their specific contributions to the research should be given.

Author Identification – ORCID

BJMBR and Scielo endorse ORCID and requires that all corresponding authors provide an ORCID iD when submitting a manuscript. We encourage coauthors to register and use their ORCID as well. 

We publish the corresponding author’s ORCID iD as well as any ORCIDs provided by coauthors if the manuscript is accepted. We participate in the auto-update feature implemented by Crossref such that when a paper is published, the authors’ ORCIDs are deposited and subsequently used to update each author’s ORCID record. 


Revised manuscripts must be accompanied by a cover letter that includes a detailed point-by-point listing as to how each of the reviewers’ comments has been addressed and describes any other changes made to the manuscript. Authors are invited to upload a copy of the original manuscript marked using Word with Track Changes, highlighting in yellow text to indicate changes and facilitate evaluation of the revisions.


BJMBR uses COPE guidelines (powered by iThenticate) to screen submitted content for originality. If plagiarism is detected during the peer review process, the manuscript may be rejected. If plagiarism is detected after publication, we may issue a correction or retract the paper, as appropriate.

We expect that editors and reviewers will be vigilant in their evaluation of BJMBR submissions and will notify the journal about any plagiarism identified.

Publication Charges

Publication charges (also referred to as “Article Processing Charges” or APCs) help BJMBR recover the costs of publication—including peer review management, journal production, and online hosting and archiving. BJMBR publishes all content Open Access and makes the content freely available online for researchers and readers to read, distribute, and reuse.

The authors are responsible for “publication charges” of all accepted papers. Publication charges will be billed to the Corresponding Author when the paper is accepted. The charge is R$3.300,00/paper for Brazilian authors and US$1,600.00/paper for authors outside Brazil and is independent of the length of the paper. The Journal does not provide reprints to corresponding authors. There is no charge for figures in color.

Please contact Reinaldo de Souza ( if you have any questions.

Cover Letter

The cover letter should also contain the following information:

  • Title of article.
  • Name(s) of all author(s).
  • Name, complete mailing address, including zip code, telephone number, fax number and e-mail of author to whom correspondence should be sent.
  • If a version of the manuscript has been previously submitted for publication to another journal, include comments from the peer reviewers and indicate how the authors have responded to these comments.
  • Papers in the area of Clinical Investigation should include a statement indicating that the protocol has been approved by the Hospital Ethics Committee (Hospital with which at least one of the authors is associated) and that written informed consent was obtained from all participants.

Text format

The text of a manuscript can only be accepted as a Microsoft Word file created with MS Word as a “doc” “docx” or “rtf” document.

  • Use preferably Arial font, 11 point type, including the title page, abstract, text, acknowledgments, references, figure legends, and tables. Each page should contain the page number in the upper right-hand corner starting with the title page as page 1.
  • Report all measurements in Système International, SI ( and standard units where applicable.
  • Do not use abbreviations in the title and limit their use in the abstract and text.
  • The length of the manuscript and the number of tables and figures must be kept to a minimum.
  • Ensure that all references are cited in the text, and listed and numbered in order that they appear in the text.
  • Generic names must be used for all drugs. Instruments may be referred to by proprietary name; the name and country or electronic address of the manufacturer should be given in parentheses in the text.

 Guidance on grammar, punctuation, and scientific writing can be found in the following sources:

  • Scientific Style and Format: The CSE Manual for Authors, Editors, and Publishers. 8th edn. Rockefeller University Press, Reston, 2006
  • Medical Style and Format. Huth EJ (Editor). ISI Press, Philadelphia, 1987, Marketed by Williams & Wilkins, Baltimore, MD.
  • Writing scientific articles like a native English speaker: top ten tips for Portuguese speakers. Clinics (Sao Paulo). Mar 2014; 69(3): 153-157. doi: 10.6061/clinics/2014(03)01

The Brazilian Journal of Medical and Biological Research follows the reference format of the Uniform Requirements for Manuscripts Submitted to Biomedical Journals, which can be found on the website of the National Library of Medicine (

The writing style should be concise and accessible. Editors will make suggestions for how to achieve this, as well as suggestions for cuts or additions that could be made to the article to strengthen the argument. Our aim is to make the editorial process rigorous and consistent, but not intrusive or overbearing. Authors are encouraged to use their own voice and to decide how best to present their ideas, results, and conclusions.

Although we encourage submissions from around the globe, we require that manuscripts be submitted in American English. As a step towards overcoming language barriers, we encourage authors to seek the assistance of professional services available on the site.


Text footnotes, if unavoidable, should be numbered consecutively in superscript in the manuscript and written on a separate page following the abstract.

Headings in text

  • Position all headings flush with the left margin.
  • Keep headings short (three or four words).
  • Use only three types of headings in the text. Clearly indicate the type of level of headings by using the following typographic conventions.
    • First-level: Only the 1st letter of the 1st word is capitalized, font size 11, bold type.
    • Second-level: Only the 1st letter of the 1st word is capitalized, font size 9, bold type.
    • Third-level: Only the 1st letter of the 1st word is capitalized, font size 11, italic type as the beginning of the first sentence of the paragraph.


Abbreviations should be kept to a minimum. Define all abbreviations upon first use in the abstract and the text. Non-standard abbreviations should not be used unless they appear at least three times in the text.

  • Explain all abbreviations in the abstract, text, figure and table legends when they first appear. Keep the number of abbreviations to a minimum.
  • Do not explain abbreviations for units of measurement [3 mL, not 3 milliliters (mL)] or standard scientific symbols [Na, not sodium (Na)].
  • Abbreviate long names of chemical substances and terms for therapeutic combinations. Abbreviate names of tests and procedures that are better known by their abbreviations than by the full name (VDRL test, SMA-12).
  • Use abbreviations in figures and tables to save space, but they must be defined in the legend.


The use of standardized nomenclature in all fields of science and medicine is an essential step toward the integration and linking of scientific information reported in published literature. We will enforce the use of correct and established nomenclature wherever possible:

We strongly encourage the use of SI units.

  • s for second
  • min for minute
  • h for hour
  • L for liter
  • m for meter
  • kDa for mass in kilodaltons
  • 5 mM rather than 5 x 10-3 M or 0.005 M

Species names (e.g., Homo sapiens), genes, mutations, genotypes, and alleles should be italicized. Use the recommended name by consulting the appropriate genetic nomenclature database, e.g., HUGO for human genes. It is sometimes advisable to indicate the synonyms for the gene the first time it appears in the text.

The Recommended International Non-Proprietary Name (rINN) of drugs should be provided.

Manuscript Categories

Research Articles

Full length Paper should clearly state its objective or hypothesis; the experimental design and methods used; the essential features of any interventions; the main outcome measures; the main results of the study; and a discussion placing the results in the context of published literature.

Short Communication is a report on a single subject, which should be concise but definitive. The scope of this section is intended to be wide and to encompass methodology and experimental data on subjects of interest to the readers of the Journal

Concepts and Comments should provides a platform for readers to present ideas, theories and views.

Case Report should have at least one of the following characteristics to be published in the Journal: 

1) special interest to the clinical research community; 

2)  a rare case that is particularly useful to demonstrate a mechanism or a difficulty in diagnosis; 

3) new diagnostic method;

4) new or modified treatment;

5) a text that demonstrates relevant findings and is well documented and without ambiguity.

Review Article, should provide a synthetic and critical analysis of a relevant area and should not be merely a chronological description of the literature.

Overview should not contain unpublished data. It presents the point of view of the author(s) in a less rigorous form than in a regular review or minireview and is of interest to the general reader.

All papers should contain:

  • Abstract of no more than 250 words,
  • key words:  no more than 6,
  • running title to be used as a page heading, which should not exceed 60 letters and spaces,
  • text: when appropriated should be divided into separate sections (Introduction, Material and Methods, Results, Discussion), without a separate section for conclusions.


Full Paper and Concepts and Comments should contain no more than 40 references.

Short Communication and Case report should contain no more than 20 references and three illustrations (figures and/or tables). 

Review Article and Overview should contain no more than 90 references.

Figures and Tables (see instructions)

Organization of the Manuscript

Most articles published in the Brazilian Journal of Medical and Biological Research will be organized into the following sections:

  • Title, Authors, Affiliations, Author for Correspondence and address
  • Abstract, Key words, Running Title
  • Introduction
  • Results
  • Material and Methods
  • Discussion
  • References
  • Acknowledgments
  • Tables with a short descriptive title and footnote legends
  • Figures with a short descriptive title, descriptive legends and uniformity in format

Continuous page numbers are required for all pages including figures. There are no specific length restrictions for the overall manuscript or individual sections. However, we urge authors to present and discuss their findings concisely. We recognize that some articles will not be best presented in our research article format. If you have a manuscript that would benefit from a different format, please contact the editors to discuss this further.

Our online submission system can support a large range of formats for text and graphics, but if you experience difficulties with the site or are concerned about the suitability of your files, please contact the Production Department of the Brazilian Journal of Medical and Biological Research (

Title Page

Title – The title should be as short and informative as possible, should not contain non-standard acronyms or abbreviations, and should not exceed two printed lines.

Single-step purification of crotapotin and crotactine from Crotalus durissus terrificus venom using preparative isoelectric focusing

Please also provide a brief “running title” of up to 60 characters and spaces.

Purification of crotapotin and crotactine

Authors and Affiliations

Initials and last name(s) of author(s) (matched with superscript numbers identifying institutions). Institution(s) (Department, Faculty, University, City, State, Country) of each author (in Portuguese if authors are from Brazil).

A.S. Aguiar1, A.R. Melgarejo1, C.R. Alves2 and S. Giovanni-De-Simone2,3

1Divisão de Animais Peçonhentos, Instituto Vital Brazil, Niterói, RJ, Brasil
2Laboratório de Microsequenciamento de Proteínas, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil
3Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Niterói, RJ, Brasil

One of the authors should be designated as the corresponding author. It is the corresponding author’s responsibility to ensure that the author list is accurate and complete. If the article has been submitted on behalf of a consortium, all consortium members and affiliations should be listed after the Acknowledgments.

Corresponding author: Name, complete mailing address, including zip code, telephone number, Fax number and E-mail of author to whom correspondence should be sent.

Key Words

A list of key words or indexing terms (no more than 6) should be included. A capital letter should be used for the first letter of each key word, separated by a semicolon. The Journal recommends the use of medical subject headings of Index Medicus for key words to avoid the use of several synonyms as entry terms in the index for different papers on the same subject. Remember, key words are used by the Scielo Database (see;articles search/subject) to index published articles.

Running title

This short title, to be used as a page heading, should not exceed 60 letters and spaces.


Since abstracts are published separately by Information Services, they should contain sufficient hard data, to be appreciated by the reader. The Brazilian Journal publishes unstructured abstracts in a single paragraph. The abstract should not exceed 250 words.

The abstract should briefly and clearly present the objective, experimental approach, new results as quantitative data if possible, and conclusions. It should mention the techniques used without going into methodological detail and mention the most important results.

Abbreviations should be kept to a minimum and should be defined in both the Abstract and text.

Please do not include any reference citations in the abstract. If the use of a reference is unavoidable, the full citation should be given within the abstract.


The Introduction should put the focus of the manuscript into a broader context. As you compose the introduction, think of readers who are not experts in this field. This should state the purpose of the investigation and justification for undertaking the research and relationship to other work in the field. An extensive listing or review of the literature should not be used. If there are relevant controversies or disagreements in the field, they should be mentioned so that a non-expert reader can delve into these issues further. The Introduction should conclude with a brief statement of the overall aim of the experiments and a comment about what was achieved.

Material and Methods

Sufficient information should be provided in the text or by referring to papers in generally available journals to permit the work to be repeated.

This section should provide enough detail for reproduction of the findings. Protocols for new methods should be included, but well-established protocols may simply be referenced. We encourage authors to submit, as separate files, detailed protocols for newer or less well-established methods. These will be linked to the article and will be fully accessable.


The results should be presented clearly and concisely. Tables and figures should be used only when necessary for effective comprehension of the data. The Results section should provide results of all of the experiments that are required to support the conclusions of the paper. There is no specific word limit for this section, but a description of experiments that are peripheral to the main message of the article and that detract from the focus of the article should not be included. The section may be divided into subsections, each with a concise subheading. Large datasets, including raw data, should be submitted as supplementary files; these are published online linked to the article. The Results section should be written in past tense. In some situations, it may be desirable to combine Results and Discussion into a single section.


The purpose of the Discussion is to identify new and relevant results and relate them to existing knowledge. Information given elsewhere in the text, especially in Results, may be cited but all of the results should not be repeated in detail in the Discussion. The Discussion should spell out the major conclusions and interpretations of the work including some explanation of the significance of these conclusions. How do the conclusions affect the existing assumptions and models in the field? How can future research build on these observations? What are the key experiments that must be done? The Discussion should be concise and tightly argued. If warranted, the Results and Discussion may be combined into one section.


When appropriate, briefly acknowledge technical assistance, advice and contributions from colleagues. People who contributed to the work, but do not fit the criteria for authors should be listed in the Acknowledgments, along with their contributions. Donations of animals, cells, or reagents should also be acknowledged You must also ensure that anyone named in the Acknowledgments agrees to being so named. Financial support for the research and fellowships should be acknowledged in this section (agency and grant number).


Figures must be submitted in high-resolution version (600 dpi). Please ensure that the files conform to our Guidelines for Figure Preparation when preparing your figures for production.

Preparing figure files for submission

The Brazilian Journal of Medical and Biological Research encourages authors to use figures where this will increase the clarity of an article. The use of color figures in articles is free of charge. The following guidelines must be observed when preparing figures. Failure to do so is likely to delay acceptance and publication of the article.

  • Each figure of a manuscript should be submitted as a single file.
  • Figures should be numbered in the order they are first mentioned in the text, and uploaded in this order.
  • Figure titles and legends should be provided in the main manuscript, not in the graphic file.
  • The aim of the figure legend should be to describe the key messages of the figure, but the figure should also be discussed in the text.
  • An enlarged version of the figure and its full legend will often be viewed in a separate window online, and it should be possible for a reader to understand the figure without moving back and forth between this window and the relevant parts of the text.
  • Each legend should have a concise title of no more than 15 words. The legend itself should be succinct, while still explaining all symbols and abbreviations. Avoid lengthy descriptions of methods. Statistical information should be given as well as the statistical tests used.
  • Arrows or letters should be used in the figure and explained in the legend to identify important structures.
  • Figures with multiple panels should use capital letters A, B, C, etc. to identify the panels.
  • Each figure should be closely cropped to minimize the amount of white space surrounding the illustration. Cropping figures improves accuracy when placing the figure in combination with other elements, when the accepted manuscript is prepared for publication. For more information on individual figure file formats, see Guidelines for figures.
  • Individual figure files should not exceed 5 MB. If a suitable format is chosen, this file size is adequate for extremely high quality figures.
  • Please note that it is the responsibility of the author(s) to obtain permission from the copyright holder to reproduce figures (or tables) that have previously been published elsewhere. In order for all figures to be open-access, authors must have permission from the rights holder if they wish to include images that have been published elsewhere in non-open-access journals. Permission should be indicated in the figure legend, and the original source included in the reference list.

Supported file types

The following file formats can be accepted.

  • TIFF (suitable for images) – with 600 dpi
  • JPG – with 600 dpi

Micrographs should be treated like photographs with the following additional guidelines

  • Electron micrographs must contain a magnification bar with its equivalence in micrometers. This information can be found on all micrographs together with the magnification size.
  • Details of any stains used and the method of preparation the sample should be given in the figure legend or in the Methods section.
  • Detailed information about the microscope used should be included in the Methods section.
  • The type of camera, photographic software and details of any subsequent image manipulation should be included in the Methods section.


  • Tables must be submitted in Word (.doc) or Excel (.xls), not as an image.
  • Tables must be numbered consecutively with Arabic numerals in the text.
  • Tables must have a concise and descriptive title.
  • All explanatory information should be given in a footnote below the table. Footnotes should be used to explain abbreviations and provide statistical information, including statistical tests used.
  • All abbreviations must be defined in this footnote, even if they are explained in the text.
  • Tables must be understandable without referring to the text.
  • Tables occupying more than one printed page should be avoided, if possible.
  • Vertical and diagonal lines should not be used in tables; instead, indentation and vertical or horizontal space should be used to group data.
  • Tables in Excel must be cell-based; do not use picture elements, text boxes, tabs, or returns in tables.


Only published or accepted manuscripts should be included in the reference list. Meeting abstracts, conference talks, or papers that have been submitted but not yet accepted should not be cited. Limited citation of unpublished work should be included in the body of the text only. All personal communications should be supported by a letter from the relevant authors. Authors are responsible for the accuracy and completeness of their references and for correct text citation. When possible, references which are easily available in English should be cited.

The BJMBR uses the numbered citation (citation-sequence) method. References are listed and numbered in the order that they appear in the text. In the text, citations should be indicated by the reference number in parentheses. Multiple citations within a single set of parentheses should be separated by commas without a space (1,5,7). Where there are more than three sequential citations, they should be given as a range (4-9).

Because all references will be linked electronically (doi), if possible, to the papers they cite, proper formatting of the references is crucial. For all references, list the first 6 authors followed by et al., Title, Journal (abbreviation), Year, Volume, Complete Pages,

The Brazilian Journal of Medical and Biological Research follows the reference format of the Uniform Requirements for Manuscripts Submitted to Biomedical Journals, which can be found on the website of the National Library of Medicine (

Please use the following style for the reference list:

Published Papers. First 6 authors followed by et al., Title, Journal (abbreviation in italics), Year, Volume, Complete Pages.

Lammers AE, Hislop AA, Flynn Y, Haworth SG. The 6-minute walk test: normal values for children of 4-11 years of age. Arch Dis Child 2008; 93: 464-468.

Zhang Q, Malik P, Pandey D, Gupta S, Jagnandan D, Belin de CE, et al. Paradoxical activation of endothelial nitric oxide synthase by NADPH oxidase. Arterioscler Thromb Vasc Biol 2008; 28: 1627-1633.

Article accepted for publication but not yet published. First 6 authors followed by et al., Title, Journal (abbreviation), Year of expected publication, (in press) at the end of the citation.

Janiszewski M, Lopes LR, Carmo AO, Pedro MA, Brandes RP, Santos CXC, et al. Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells. J Biol Chem 2005 (in press).

Internet Communication. Ensure that URLs are active and available.

Brasil. Associação Brasileira para o Estudo da Obesidade: pesquisa da população brasileira. Accessed February 22, 2008.

Information behaviour of the researcher of the future, CIBER Report.

CAPES Statistics. Accessed March 16, 2006.

CNPq Plataforma Lattes, “Investimentos do CNPq em CT&I”. Accessed March 16, 2006.

Audiovisual Material

Physician’s Desk Reference (PDR). Release 2003.1AX. [CD-ROM]. Montvale: Thomson PDR; 2003.

Computer Program

Dean AG, Dean JA, Coulombier D, Brendel KA, Smith DC, Burton AH, et al. Epi info, version 6.04: a word processing database and statistics program for public health on IBM-compatible microcomputers. [Computer program]. Atlanta: Centers of Disease Control and Prevention; 1998.

Statistical Package for the Social Sciences (SPSS). Version 12.0. [Computer program]. Chicago: SPSS Inc.; 2006.


Larsen CE, Trip R, Johnson CR. Methods for procedures related to the electrophysiology of the heart. Patent No. 5.529.067. Novoste Corporation; 1995.

Book, Whole. Authors, Book title, Edition, City, Publisher, Year.

American College of Sports Medicine. Diretrizes do ACSM para os testes de esforço e sua prescrição. Rio de Janeiro: Guanabara Koogan; 2007.

Book, Chapter. Authors, Chapter Title, Editors, Book title, Edition, City, Publisher, Year, Pages of citation.

Kronfol A. Behavioral effects of cytokines: a psychiatrist’s perspective. In: Plotnikoff NP, Faith RE, Murgo AJ, Good RA (Editors), Cytokines, stress and immunity. London: CRC Press; 2007. p 1-16.

Kintzios SE. What do we know about cancer and its therapy? In: Kintzios SE, Barberaki MG (Editors), Plants that fight cancer. New York: CRC Press; 2004. p 1-14.


WHO (World Health Organization), IPCS (International Program in Chemical Safety). Environmental health criteria: 118 Inorganic mercury. Geneva: World Health Organization; 1991.

National Commission on Sleep Disorders Research. Wake up America: a national sleep alert. Washington: Government Printing Office; 1993.

National Heart Lung and Blood Institute. Global Initiative for Asthma (GINA). Global strategy for asthma management and prevention: NHLBI/WHO Workshop Report. Bethesda: National Institute of Health. National Heart, Lung and Blood Institute publication No. 02-3659; 2006.


Joselevitch C. Visão no ultravioleta em Carassius auratus (Ostariophysi, Cypriformes, Cyprinidae): estudo eletrofisiológico do sistema cone – células horizontais. [Master’s thesis]. São Paulo: Instituto de Psicologia, USP; 1999.

Conference, Symposium Proceedings. Cite papers only from published proceedings.

Hejzlar RM, Diogo PA. The use of water quality modelling for optimising operation of a drinking water reservoir. Proceedings of the International Conference Fluid Mechanics and Hydrology. 1999 Jun 23-26; Prague. Prague: Institute of Hydrodynamics AS CR; 1999. p 475-482.

Unpublished results”, “Personal communication” and “Submitted papers”. Reference should appear in the text with the individual name(s) and initials and not in the reference list.

(Santos CS, da-Silva GB, Martins LT, unpublished results).

It is assumed that the author has obtained permission from the source when “personal communication” is cited.